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Most people feel only a small pinch during the needle insertion and a light warmth as the drip starts
(and GO P53!!!)
The GST tag can easily be removed following purification by addition of a protease if a suitable protease-cleavage site has been inserted between the GST-tag and the protein of interest (which is usually included in many commercially available sources of GST-tagged plasmids)
In a three-hour Senate hearing this week, Dr
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