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But when you flood the system with exogenous glutathione, especially in reduced form, you're not just boosting antioxidant capacity
Skin Rejuvenation and Anti-Aging Protocol Dose: 1 to 2 mg per day Route: Subcutaneous (abdomen, thigh, or upper arm) Frequency: Daily, 5 days on, 2 days off Cycle: 8 to 12 weeks on, 4 weeks off Expected results: 4 to 8 weeks for visible improvement Best for: Fine lines, skin elasticity, skin density, overall skin quality In a 12-week facial cream study (human trial, 71 women), 70% showed increased collagen production with GHK-Cu, compared to 50% with vitamin C and 40% with retinoic acid (Pickart et al., 2015)
After incubation for 15 min at 30 C, 1,000 rpm using a 3 mm shaking diameter, cell debris was removed by centrifugation (4,400 g at 4 C for 30 min) and the supernatants were analysed by HPLCMS at two different dilutions (undiluted and four times diluted with 67% methanol, 0.2% hydrochloric acid in H 2 O)
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