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20 G i/o family proteins inhibit adenylate cyclase (AC) activity, leading to reduced intracellular cAMP levels and therefore inhibiting the protein kinase A (PKA) signaling pathway

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Bottom left: VX exerts toxicity through irreversible inhibition of acetylcholinesterase (AChE), causing accumulation of acetylcholine (ACh) at synapses and resulting in cholinergic overstimulation