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The cells were washed, resuspended in SC-trp with H 2 O 2 (Wako, 084-07441), tert -butyl hydroperoxide (Wako, 026-13451), menadione (Wako, 132-08132), or diamide (Sigma-Aldrich, D3648), and cultured at 30C for 1 h
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To become useful again, the body must use a specific enzyme called glutathione reductase to "recycle" it back into the reduced state
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