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Sections were subjected to a third Citra antigen retrieval, followed by guinea pig anti-insulin (Agilent/Dako, A0564) (1:1,000, 30 minutes), biotinylated goat anti-guinea pig IgG (Vector, BA-7000) (30 minutes), and Warp Red chromogen (Biocare Medical, WR806) (1 minute)
5b and 6a are provided in Supplementary Figs
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