To construct the sorting vector pLKO.1-GST DAF for shRNA gene knockdown experiment, we amplified the coding sequence of N-signal-GST-EGFP-GPI DAF from the pEGFP-GST-GPI DAF plasmid and joined it with FastDigest KpnI/FastDigest BamHI (Thermo Fisher) linearized pLKO.1 vector using ClonExpress II One Step Cloning Kit
19(8):969-988 Peptide Sequence: Gly-His-Lys(Cu2+) Hong Y, Downey T, Eu KW, Koh PK, Cheah PY (Department f Colorectal Surgery, Singapore General Hospital, Singapore, 169608,Singapore) "A 'metastasis-prone' signature for early-stage mismatch-repair proficient sporadic colorectal cancer patients and its implications for possible therapeutics" In Clin Exp Metastasis
EBioMedicine 50:274289 Jimnez-Villegas J, Kirby J, Mata A et al (2022) Dipeptide repeat pathology in C9orf72-ALS is associated with redox, mitochondrial and NRF2 pathway imbalance
doi: 10.3389/fnut.2023.1155306, 113