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doi: 10.1136/gut.48.2.206 51
Realtime quantitative PCR (RTqPCR) Total RNA of ovarian tissues was extracted according to the instructions of a SPARKeasy Improved Tissue/Cell RNA Extraction Kit (Sparkjade, AC0202, Shandong, China), and cDNA was generated through reverse transcription by using SPARKscript II RT Plus (Sparkjade, AG0304, Shandong, China)
Definition The term colitis defines abdominal pain or endoscopic/radiologic evidence of colonic inflammation [7]
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