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Subsequent studies demonstrated that apelin signaling is highly context dependent, with differential coupling to G_i, G_q, and -arrestin pathways depending on ligand structure, receptor density, and tissue environment ( Translationally, the apelinAPJ system illustrates a broader challenge for metabolic peptides with cardiovascular overlap: indication-specific development is likely required

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Chronic daily treatment with acyl-GIP reduced body weight and food intake in control DIO Gipr AP-GFP mice, associated with a significant reduction in fat mass