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Osmotic pump implantation On Day 0, all mice were implanted subcutaneously with an Alzet Model 2004 minipump, where the stainless-steel cannula was replaced with PEEK tubing (DURECT Corporation, Cat# 0002496) to allow for further body composition scanning
For negative controls, the staining solution included 5 mM serine (Sigma-Aldrich, S4311) and 10 mM sodium borate (Sigma-Aldrich, 71997)
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