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Samples were transferred to Miro5 buffer (pH 7.1, 110 mM sucrose, 60 mM potassium lactobionate, 20 mM taurine, 10 mM monobasic potassium phosphate, 3 mM magnesium chloride, 20 mM HEPES, 0.5 mM EGTA, 1 mg/mL BSA) and washed for 3 10 min on a rocker at 4 C

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Purpose of Urine Glucose Test: To monitor blood glucose levels in people with diabetes To screen for diabetes in people who may be at risk for the disease To detect other health conditions such as kidney disease, liver disease, or pancreatic disorders that may affect glucose metabolism To assess the effectiveness of diabetes treatment To identify gestational diabetes during pregnancy To check for urine glucose as a part of routine medical examination Why Get Tested: To diagnose diabetes or prediabetes To monitor blood glucose levels in people with diabetes To screen for diabetes in people who may be at risk for the disease To assess the effectiveness of diabetes treatment To detect other health conditions such as kidney disease, liver disease, or pancreatic disorders that may affect glucose metabolism To identify gestational diabetes during pregnancy To check for urine glucose as a part of routine medical examination When to Get Tested: If you have symptoms of diabetes such as frequent urination, increased thirst, blurred vision, or fatigue If you have a family history of diabetes If you are overweight or obese If you are pregnant, to screen for gestational diabetes If you have a history of high blood glucose levels If you are on medications that affect blood glucose levels As part of routine medical examination, especially if you are at risk for diabetes