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2.15 Real-time quantitative PCR Total RNA was extracted from aortic tissue and endothelial cells using TRIzol reagent (Takara Bio Inc., 9108), following the manufacturers instructions
Caution: If any side effects occur, always discuss with a doctor, as they can assess their severity, determine whether treatment adjustments are needed
doi:10.1016/j.jconrel.2011.01.030
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