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This was consistent with our findings, where we found nearly an absence of the hepatic tissue content of Bcl-2 in the MTX + CCl 4 group, while a significant protein expression of Bcl-2 protein was observed in the normal group, and the CCl 4 (8 weeks) group showed weak Bcl-2 protein expression (Song et al., 2020)

Quantitative reverse transcription PCR (RT-qPCR) Total RNA was isolated from cells or tumor tissues using an RNA-easy Isolation Reagent (R701-01, Vazyme, China)

[28] [29] Testosterone promotes prostate cell proliferation, [30] but relatively low levels of serum testosterone are found in patients with BPH