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Finally, the slides were fixed with Antifade Mounting Medium (S2100, Solarbio), and images were acquired using a confocal laser scanning microscope (LSM900, ZEISS)
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PCR was performed to amplify the libraries (Vazyme, TD601) using the following PCR conditions: 72 C for 3 min
Concentration Options Storage Conditions Store the reconstituted Fragment 176-191 solution under refrigerated conditions at 2-8C for short-term stability up to 4 weeks
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