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Reconstitute each vial with a fixed volume of bacteriostatic water so the concentration lands where the protocol expects
The cells were then fixed with frozen methanol for 15 min at 4 C and washed with PBS three times, followed by the addition of DAPI (4,6-diamidino-2-phenylindole, Beyotime Biotechnology Co
B6 and ATP for training muscles that improves the equine performance
Freshly purified proteins were subsequently analysed in steady-state kinetic measurements using GSSCys and HEDS as alternative disulfide substrates
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