Deficiency in glutathione or its cofactors, including selenium and riboflavin, may contribute to the progression of autoimmune thyroid conditions such as Hashimotos thyroiditis (1,2)
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The cell pellet was thawed, homogenized, and lysed in a buffer containing 20 mM HEPES pH 7.5, 50 mM NaCl, 2 mM MgCl 2 , and complete protease inhibitor cocktail tablet (Roche), and the membranes were collected by centrifugation at 40,000 g
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