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To explore the interactions between AvrPto and neighboring immune system proteins, the authors designed four constructs: AvrPto_BirA (fusion of AvrPto with the N-terminus of BirA), MP_BirA (fusion of an 8-amino acid AvrPto myristoylation/palmitoylation (MP) motif with the N-terminus of BirA), BirA alone, and an empty vector (EV) control
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