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Some 100 L of lysates were then incubated with 10 mM dithiothreitol (final concentration, dissolved in water) on a thermomixer at 56 C, at 1,000 rpm for 30 min, followed by an incubation with 20 mM iodoacetamide (final concentration, dissolved in water) at 25 C, at 1,000 rpm for another 30 min in the dark

World Obesity Atlas 2023 (Global Obesity Observatory, 2023)

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High-Performance Liquid Chromatography serves as the premier analytical method for evaluating chemical purity