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To quantify NHO volumes, the region of interest (ROI) was drawn around the muscles containing NHO, and these were then carefully checked from three dimensions to ensure adjacent long bones were not included in the ROI
Apart from Nlrp3 inflammasome, mitogen-activated protein kinases (MAPK) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-B) are involved in the pathogenesis of chronic inflammation (47)
After reconstitution, aliquot and store at 20 C to preserve structural stability
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