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Proteins were denatured in sample buffer and equal amounts were separated on SDS-PAGE and transferred to PVDF membrane (BioRad Laboratories, CA, USA)
Duration of Effects Immediate Effects: Increased energy and improved well-being can be felt almost instantly and may last for several days
Manufactured in the USA and supplied in a sealed 30 mL vial
We observed reduced weight loss in Gdf15 -KO mice on day 1, although the difference diminished by day 7 (Extended Data Fig
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