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Mice were tested for social interaction (SI) 24 h after the last social defeat by first allowing 2.5 min for the test mouse to explore an arena containing a plexiglass wire mesh cage centered against one wall of the arena (target absent)

Additionally, he advises them to watch for warning signs

The most common b12 injections side effects and safety considerations are mild: slight soreness at the injection site, occasional brief nausea, or a short-lived headache

After washing several times in 0.01 M PBS, the samples were treated with anti-rabbit IgG antibodies (Thermo Fisher Scientific) in 0.01 M PBS at 22 2 C for 1 h