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THP-1 cells were seeded at 2 10 5 cells per well in complete RPMI 1640 medium with 100 nM phorbol 12-myristate 13-acetate (PMA) (Cayman) and differentiated into macrophages for 3 days, then rested in PMA-free medium for 16 h before infection
10.1038/nrc1751 Nat
TB-500 (Thymosin Beta-4) is more systemic, supporting whole-body recovery and flexibility
PubMed For in vitro research use only
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