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The slices were incubated for at least 1 hour at 34C in ACSF composed of 126 mM NaCl, 2.8 mM KCl, 26 mM NaHCO 3 , 1.25 mM NaH 2 PO 4 , 1.2 mM MgSO 4 , 2.5 mM CaCl 2 , and 5 mM glucose (pH 7.3), while being continuously bubbled with 95% O 2 and 5% CO 2

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NAC offers important protection to the brain, heart and lungs, but because of its unique detoxifying role, NAC provides targeted protection to the hardworking cells of the liver and kidneys making it a sensible part of any internal cleanse or detoxification program

All of them were kept at 4 C for several days without a noticeable change in the analytes content