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Then, prepared cell lysates were plated on a 96-well plate (Nunc) overnight at 4 C and incubated with 5% BSA PBST (NaCl 137 mM, KCl 2.7 mM, Na 2 HPO 4 10 mM, KH 2 PO 4 1.8 mM, 0.1% Tween 20) for 2 h at room temperature (RT)