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Digested samples were desalted using a C18 spin column (Pierce C18 Spin, #89873) according to manufacturer instructions prior to resuspension in EPPS buffer and CH 3 CN for TMT labeling as described above

However, clinical evidence supporting broad wellness claims remains limited, with most robust data emerging from specific disease or metabolic contexts rather than general population use

Most clinical guidelines and obesity specialists recommend GLP-1 medications as long-term or indefinite treatments for obesity similar to how blood pressure or cholesterol medications are used chronically

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