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Secondary carnitine deficiencies are most commonly the result of trapping of carnitine in fatty acylcarnitine due to defects in mitochondrial fatty acid -oxidation enzymes such as is the case in medium-chain acyl-CoA dehydrogenase deficiency (MCADD)

Following fixation, cell cultures were first incubated with an SGC marker (glutamine synthesis rabbit IgG, Abcam ab73593) and then incubated with an IgG marker using anti-human IgG (AF594, Thermo Fisher A11014) and anti-rabbit IgG antibody (AF488, Thermo Fisher A11008)

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Other signaling pathways involved in cell dysfunction mTOR can phosphorylate AGC family of protein kinases, regulating cell survival and proliferation