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Total RNA was extracted from the colon MC and normal colon tissues using TRIzol reagent (Gibco Life Technologies, Carlsbad, CA, USA) according to the manufacturer's instructions
Products are not approved by the FDA or any regulatory authority for human consumption, therapeutic, or cosmetic use.
For the second group, those with a pre-existing substance use disorder, the researchers tracked which of the 81,617 participants experienced drug-related emergency department visits, hospitalization, mortality, overdose, or suicidal ideation or attempt
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