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Vitamin B12 injections are very safe and well tolerated
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Because each sequence in the initial alignment has a length of 30 amino acids and each position can be mutated to one of 19 amino acids (20 if the position is gapped), this gives 570 single-step mutants in the first generation for each sequence in the training set, that is, 71,304 sequences, reducing to 69,639 sequences after removing duplicates
While the procedure itself is relatively quick, lasting around 30 minutes, it is normal to experience some mild swelling, redness, or bruising at the injection site
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