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IGF-I signaling in the osteoblastic lineage The osteoblastic lineage begins with mesenchymal progenitors that progress through stages of preosteoblasts (Pre-OB), mature osteoblasts (OB) and, finally, osteocytes (OCY, Figure 2)
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For lipidomic analysis, liquid cultures in tween-free 7H9 medium supplemented with albumin, dextrose and sodium chloride were transferred to nitrocellulose filter-paper discs using vacuum filtration and grown on solid agar media 67
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