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For silencing FOXO4, specific small interfering RNA (siRNA) was employed, with a negative control siRNA, and transfection was conducted using Lipofectamine 3000 [2]
It is prepared by a licensed pharmacist rather than a mass-market manufacturer
When researchers blocked PDE4, an enzyme that normally breaks down that signal, more cells stayed responsive for longer
The researchers observed that FOXO4 transitions into the nucleus following the induction of the senescence in the cell
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