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Two experimental wounds, each 2 cm x 2 cm, were created on either side of the dorsal midline, approximately 4 cm caudal to the scapula

For Oil Red O Staining, liver tissues were frozen in Tissue-Tek CRYO-OCT (Fisher Scientific) and cryostat tissue sections were cut at 7 m thicknesses, fixed with 10% formalin for 10 min, and stained following an Oil Red O procedure

Our biochemical and transcriptional profiling of murine liver tumors identified six pathways that are coordinately altered in vivo (Fig 1B and Appendix Figs S1S6)

O consumo noite, principalmente antes de dormir, no recomendado por se tratar de um estimulante, o que pode causar insnia com o uso continuado