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The present invention provides a pharmaceutical composition comprising semaglutide or a salt thereof, a bioavailability improver, and sustained-release microspheres made of a biodegradable polymer, exhibiting high bioavailability and stable drug release characteristics over a long period of time, and a pharmaceutical composition comprising semaglutide or a salt thereof and sustained-release microspheres made of a biodegradable polymer, exhibiting safe drug release characteristics with high drug content and low initial release, and a method for producing the sustained-release microspheres

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