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AMPK phosphorylation was suppressed, and autophagy activity was inhibited in high-glucose cultured human renal proximal epithelial cells (HRPTCs) and STZ-induced diabetic nephropathy mice

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One milliliter of a PBS-based reaction mixture containing propidium iodide (20 g/mL), RNase (0.2 g/mL), and 0.1% Triton X-100 (Sigma) was added to the cell samples at a density of 1 10 6 cells/mL and incubated in the dark for 40 min before a DNA content analysis was conducted through flow cytometry