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10.1186/s12933-021-01216-7 12
Primary antibodies were applied sequentially: NLRP3 (GB114320, 1:400, servicebio), GSDMD (GB154198, 1:500, servicebio), Caspase-1 (GB15383, 1:2000, servicebio), and apoptosisassociated specklike protein containing a CARD (ASC, GB153966, 1:500, ServiceBio) at room temperature overnight at 4C
All primary antibodies were used at a dilution of 1:1,000, except -actin (1:5,000)
The formulation is commonly used both as a standalone therapy and as part of combination IV drips to amplify wellness and aesthetic outcomes
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