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The redox electrode was calibrated using ZoBells solution (2.64 g K 4 [Fe(CN) 6 ].3H 2 O and 2.06 g K 3 [Fe(CN) 6 ].H 2 O dissolved in 500 mL pH = 7 0.15 mol/L Na 2 HPO 4 /KH 2 PO 4 buffer)
This distinction is particularly important for engineering because modifying downstream responsive genes often produce transient phenotypic effects, whereas manipulation of upstream regulatory hubs is more likely to generate coordinated and durable metabolic responses
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