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In brief, tissue sections were deparaffinized and rehydrated using a gradient method for antigen retrieval
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Stock solutions prepared in DMSO are stable for 3 to 4 weeks when stored at 28C, or up to 6 months when aliquoted and stored at -20C or -80C
Because GHK-Cu operates through cumulative signaling rather than peak concentration, researchers commonly build in an escalation: a lower dose in the first half of a cycle, a higher dose in the second half, followed by a deliberate rest period before starting again
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