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(G) The levels of catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and malondialdehyde (MDA) were determined by biochemical assay kits
Cells were fixed with 2% paraformaldehyde (15 min), permeabilized with 0.1% Triton X-100 (10 min) and blocked with 2% BSA (1 h)
Ambition must be balanced with safety because overuse can reduce rewards and pose health hazards
Five unique GSTP1 -specific shRNA plasmids (clones TRCN0000083 773 , TRCN0000083 774 , TRCN0000083 775 , TRCN0000083 776 and TRCN0000083 777 ) and a negative control plasmid (empty vector control, SHC001) were purchased as glycerol stocks and plasmid DNA extracted using plasmid DNA maxi prep kits (Qiagen, Manchester, UK) according to the manufacturers instructions
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