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As an initial application of the method, we generated a library of all possible single-residue substitutions to a 60-mer VAMP1 sequence and assessed the cleavage activities of wild-type BoNT/X and BoNT/F proteases on this library (Extended Data Fig
Similar results were observed in an experiment involving a germ-free mouse strain, where the animals transplanted with microbiome isolated from depressed patients showed an increase in immobility time in the forced swim test and a decreased proportion of central motion distance in the open field test, compared to mice transplanted with bacteria obtained from healthy control subjects [101]
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