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Both times are highly effective
Cell viability was assessed using the Cell Counting Kit-8 (CCK8) assay, measuring metabolic activity as an indicator of cell health
Each location contains sufficient subcutaneous fat to safely accommodate the 0.5 mL standard dose, though abdominal and thigh sites tend to offer the most consistent absorption patterns due to their larger surface area and predictable blood flow
If they are still partially frozen or cold to the touch, the medication is likely fine
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