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This strategy ensures high specificity for glutathione-modified peptides while maintaining sensitivity across a broad dynamic range
After completion, as monitored by UPLC and ESI-LCMS, the reaction mixture was diluted with buffer A, filtered and purified directly by preparative RP-HPLC, eluting with a linear gradient (see the Peptide cleavage and purification section)
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Each association suggests an independent mechanism by which the de novo thymidylate synthesis and remethylation pathways may be influenced by UV environment
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