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In practical terms, this means aged fibroblasts exposed to GHK-Cu begin behaving more like younger fibroblasts producing more matrix proteins and degrading fewer

Quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) was performed using a QuantiTect SYBR-Green RT-PCR kit (Qiagen, Venlo, The Netherlands) and a CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, USA)

We define longevity as a function of two things: lifespan (how long you live) and healthspan (how well you live)