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Crypts ( N = 500) per well were seeded in 50 l Matrigel (BD, 356231) on a pre-warmed 24-well plate and allowed to solidify for 10 min at 37 C, after which 500 l IntestiCult Growth Medium supplemented with 100U/ml penicillin and 100 g/ml streptomycin (Biochrome, 0257F) was added
Stanowi doskonay materia odniesienia w testach takich jak: Analizy ilociowe HPLC (badanie profili elucji mieszanin wieloskadnikowych i zachowania stabilnoci obu wiza peptydowych)
During periods when brand-name tirzepatide has been on the FDA shortage list, compounding has been legally permitted
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