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10.3389/fnagi.2017.00345 109 NeuS

Islets were separated from pancreatic tissue using a histopaque gradient and allowed to recover in RMPI (11.1 mM glucose, 10% FBS [Thermo Fisher Scientific, 10437028], 1% pen/strep) overnight before experiments were performed

This builds on what we already know: menopause fundamentally alters metabolism

To examine the similarity of peptides predicted by different models within each potency profile, we used PCA, considering the 500 one-hot-encoded sequences generated across all five compared models, the wild-type peptideshGCG and hGLP-1and their single-step mutants (551 hGCG and 570 hGLP-1), such that the projection was computed for an array [1,621 21 L ]