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Conclusion: ASK1 inhibition suppresses profibrogenic release of mtDNA from dying hepatocytes in p38-dependent manner and protects from liver fibrosis
Immunohistochemistry We prepared 10-m-thick colonic and pancreatic sections and 25-m-thick brain sections following fixation in 4% paraformaldehyde (wt/vol.) overnight at 4C and a sucrose gradient (15% [wt/vol.] for 6 h, 30% overnight) as previously described [4, 21]
Beyond neurons, SPHKAP is also expressed in metabolically relevant tissues, including pancreatic islets and intestinal K- and L-cells, and has been previously shown to play an important role in both incretin secretion and incretin-dependent glucoregulation 35, 36
