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The sections were then washed three times in TNT buffer (0.1 M TrisHCl, 0.15 M NaCl, 0.05% Tween20, pH 7.5) for 5 min, and treated with secondary antibodies
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N-acetylcysteine at doses among 2,4003,600 mg/day have been used for the treatment of cocaine, nicotine, alcohol, and cannabis relapse in humans (Duailibi et al., A possible depressant effect in the animals in the present study, in which the rats were single housed without environmental enrichment, is unlikely as a cause of neuroinflammation, because as shown in Figure 6, control animals housed individually and receiving water as the only fluid do not show astrocyte or microglial abnormalities
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