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The present study also explores the possibility that the oxidized inactive form of e-GST may be present in blood, given that two critical cysteines, Cys47 and Cys101 (the latter corresponding to Cys103 in pig e-GST) are strictly conserved in all mammals tested, and that they form a disulfide both in vivo and in vitro as proved for the human enzyme
doi: 10.1186/s12958-019-0526-3 76 YoonK.KimN
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With CellTox Green Dye present, there was no significant difference in total glutathione levels detected, indicating no adverse effect of multiplexing these two assays (compare green and gray bars, Figure 5, Panel B)
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