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Su H, Newman E
Serum limitation-induced senescent fibroblasts model and effect of FOXO4-DRI Passage 3 fibroblasts derived from keloids and normal skins were cultured in a Dulbeccos Modified Eagles medium (DMEM, Hyclone, Logan, Utah, USA) containing 10% fetal bovine serum (FBS, Gibco, Grand Island, NY, USA) to 90% confluence, and then cultured in a DMEM containing 2% FBS for 3 days to construct a serum limitation-induced senescent fibroblast model
Increased platelet activation, characterized by elevated levels of platelet-derived thromboxane B2, -thromboglobulin, platelet-activating factor, and P-selectin, along with decreased ADAMTS-13 and elevated VWF antigen and activity, further support the role of platelet-mediated thrombosis in the development of DCI (8084)
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