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Escherichia coli were harvested by centrifugation and resuspended in HEPES buffer (20 mM HEPES, 300 mM NaCl, pH 7.4)
While most animals can convert d-glucose into l-ascorbic acid, humans and other primates, guinea pigs, some fish and birds, and insects are unable to produce ascorbic acid endogenously
Researchers should ensure their storage facilities meet the temperature requirements and have backup power systems to prevent temperature fluctuations that can compromise the compound
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