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[47] In industry-funded preliminary trials, tirzepatide showed minor improvement of reductions (2.01%2.30% depending on dosage) in glycated hemoglobin tests relative to the injected GLP-1 analog semaglutide (1.86%)
The powder was suspended in 2 ml of histone lysis buffer (10 mM Hepes [pH 7.9], 1.5 mM MgCl 2 , 10 mM KCl in Milli-Q H 2 O with the addition of 0.6 mM DTT, sodium butyrate, and 1x cOmplete protease inhibitor EDTA-free immediately before use) and incubated on a roller for 30 min at 4C
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