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Finally, we performed high-resolution TEM imaging for precise quantification of mitochondrial area and number of cristae per mitochondria in ultrathin (70 nm) sections on resin-embedded Control versus SPHKAP RNAi-treated WT INS-1 832/3 cells, as well as INS-1 832/3 GLP-1R KO cells, both under vehicle conditions and following exendin-4 stimulation

A combination approach could address both upstream metabolic drivers and downstream pathological consequences

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The new model will include standardized coverage criteria as well as lifestyle supports and is voluntary for state Medicaid programs, Medicare Part D plans, and manufacturers