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The recommended dilution factor for different samples is as follows (for reference only): Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)

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An additional constraint could arise from the fact that while ferrostatin-1 has also previously shown efficacy in inhibiting ferroptosis in other human organoid models, such as bronchial [56] and gut crypt organoids [57], its utility is hindered by its solubility issues

However, it remains unclear whether the inhibitory effect was due to the entire BLG protein or a specific component of it